NDC80 Antibody (9G3.23)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-338

Novus Biologicals, part of Bio-Techne
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NB100-338
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Hamster, Marsupial

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Proximity Ligation Assay, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 9G3.23

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDC80 Antibodies »

Product Specifications

Immunogen

Human NDC80 protein consisting of amino acids 56-642.

Reactivity Notes

Kangaroo rat (100%).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDC80 Antibody (9G3.23)

Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
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Applications for NDC80 Antibody (9G3.23)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1-5 ug/ml

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.6 using NB100-338 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDC80

Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Alternate Names

HEC1KNTC2, HECRetinoblastoma-associated protein HEC, Highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, highly expressed in cancer, rich in leucine heptad repeats (yeast), hsHec1, hsNDC80, kinetochore associated 2, Kinetochore protein Hec1, kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3

Entrez Gene IDs

10403 (Human)

Gene Symbol

NDC80

UniProt

O14777 (Human)

Additional NDC80 Products

Product Documents for NDC80 Antibody (9G3.23)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NDC80 Antibody (9G3.23)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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