NDE1 Antibody (2G11-1C11)

Novus Biologicals, part of Bio-Techne | Catalog # H00054820-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G11-1C11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NDE1 Antibodies »

Product Specifications

Immunogen

NDE1 (AAH01421, 1 a.a. ~ 335 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC

Reactivity Notes

Human. Other species not tested.

Specificity

NDE1 - nudE nuclear distribution gene E homolog 1 (A. nidulans)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NDE1 Antibody (2G11-1C11)

Western Blot: NDE1 Antibody (2G11-1C11) [H00054820-M01]

Western Blot: NDE1 Antibody (2G11-1C11) [H00054820-M01]

Western Blot: NDE1 Antibody (2G11-1C11) [H00054820-M01] - NDE1 monoclonal antibody (M01), clone 2G11-1C11 Analysis of NDE1 expression in Hela.
Immunocytochemistry/ Immunofluorescence: NDE1 Antibody (2G11-1C11) [H00054820-M01]

Immunocytochemistry/ Immunofluorescence: NDE1 Antibody (2G11-1C11) [H00054820-M01]

Immunocytochemistry/Immunofluorescence: NDE1 Antibody (2G11-1C11) [H00054820-M01] - Analysis of monoclonal antibody to NDE1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: NDE1 Antibody (2G11-1C11) [H00054820-M01] - Detection limit for recombinant GST tagged NDE1 is approximately 0.03ng/ml as a capture antibody.

Applications for NDE1 Antibody (2G11-1C11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDE1

The NDE1 gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This pr

Alternate Names

FLJ20101, HOM-TES-87, nuclear distribution protein nudE homolog 1, NudE, nudE nuclear distribution gene E homolog 1 (A. nidulans), NUDE1, rat homolog

Entrez Gene IDs

54820 (Human)

Gene Symbol

NDE1

OMIM

609449 (Human)

UniProt

AAH01421 (Human)

Additional NDE1 Products

Product Documents for NDE1 Antibody (2G11-1C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NDE1 Antibody (2G11-1C11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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