NDUFB11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12681

Novus Biologicals, part of Bio-Techne
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NBP3-12681-50ul
NBP3-12681-100ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NDUFB11 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human NDUFB11 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for NDUFB11 Antibody

Western Blot: NDUFB11 Antibody [NBP3-12681]

Western Blot: NDUFB11 Antibody [NBP3-12681]

Western Blot: NDUFB11 Antibody [NBP3-12681] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:19 kDa
Immunocytochemistry/ Immunofluorescence: NDUFB11 Antibody [NBP3-12681]

Immunocytochemistry/ Immunofluorescence: NDUFB11 Antibody [NBP3-12681]

Immunocytochemistry/Immunofluorescence: NDUFB11 Antibody [NBP3-12681] - Staining of NDUFB11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NDUFB11 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP3-12681]

Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP3-12681]

Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP3-12681] - Staining of human NDUFB11 in human small intestine with rabbit polyclonal antibody at 1:500 dilution.
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Applications for NDUFB11 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFB11

NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain, the transfer of 2 electrons from NADH to ubiquinone, coupled to the translocation of 4 protons across the membrane (Carroll et al., 2002 (PubMed 12381726)).(supplied by OMIM)

Alternate Names

CI-ESSS, complex I NP17.3 subunit, Complex I-ESSS, ESSS, FLJ20494, MGC111182, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11, 17.3kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial, NADH-ubiquinone oxidoreductase ESSS subunit, Neuronal protein 17.3, Np15, NP17.3, p17.3

Gene Symbol

NDUFB11

Additional NDUFB11 Products

Product Documents for NDUFB11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFB11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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