NDUFB7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97854

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Cynomolgus Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NDUFB7 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human NDUFB7 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for NDUFB7 Antibody

Western Blot: NDUFB7 Antibody [NBP2-97854]

Western Blot: NDUFB7 Antibody [NBP2-97854]

Western Blot: NDUFB7 Antibody [NBP2-97854] - Anti-NDUFB7 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 19 kDa
Immunocytochemistry/ Immunofluorescence: NDUFB7 Antibody [NBP2-97854]

Immunocytochemistry/ Immunofluorescence: NDUFB7 Antibody [NBP2-97854]

Immunocytochemistry/Immunofluorescence: NDUFB7 Antibody [NBP2-97854] - Immunofluorescence staining of NDUFB7 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NDUFB7 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854]

Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854]

Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854] - Immunochemical staining of human NDUFB7 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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Applications for NDUFB7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFB7

NDUFB7 is encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternate Names

B18, Cell adhesion protein SQM1, CI-B18NADH-ubiquinone oxidoreductase B18 subunit, complex I B18 subunit, Complex I-B18, MGC2480, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (18kD, B18), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Gene Symbol

NDUFB7

Additional NDUFB7 Products

Product Documents for NDUFB7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFB7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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