Ndufs1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31142

Novus Biologicals, part of Bio-Techne
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NBP1-31142
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (96%), Chimpanzee (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ndufs1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%), Chicken (86%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ndufs1 Antibody

Western Blot: Ndufs1 Antibody [NBP1-31142]

Western Blot: Ndufs1 Antibody [NBP1-31142]

Western Blot: Ndufs1 Antibody [NBP1-31142] - Effect of BGP-15 treatment on the electron transport chain complex I and III proteins in NRCMs. Western blot analysis of UQCRC1 (NBP2-03825) and Ndufs1 (NBP1-31142) proteins is shown. GAPDH was used as a loading control. Control group: cells without any treatment; BGP-15 group: cells with only 50uM BGP-15 for 0.5 hours; H2O2 group: cells with 150uM H2O2 for 0.5 hours; H2O2+BGP-15 group: cells with 150uM H2O2and 50uM BGP-15 for 0.5 hours.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33728024/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-31142]

Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-31142]

Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-31142] - Ovarian carcinoma. NDUFS1 antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: Ndufs1 Antibody [NBP1-31142]

Western Blot: Ndufs1 Antibody [NBP1-31142]

Western Blot: Ndufs1 Antibody [NBP1-31142] - 50 ug mouse brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:500.
View 2 more images

Applications for Ndufs1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ndufs1

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Long Name

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Alternate Names

CI-75kD, Complex I-75kD, EC 7.1.1.2

Gene Symbol

NDUFS1

Additional Ndufs1 Products

Product Documents for Ndufs1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ndufs1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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