Ndufs1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52060

Novus Biologicals, part of Bio-Techne
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NBP1-52060
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Ndufs1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TEKSATYVNTEGR corresponding to internal region according to NP_004997.4.

Epitope

C-TEKSATYVNTEGR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ndufs1 Antibody

Western Blot: Ndufs1 Antibody [NBP1-52060]

Western Blot: Ndufs1 Antibody [NBP1-52060]

Western Blot: Ndufs1 Antibody [NBP1-52060] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-52060]

Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-52060]

Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-52060] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Ndufs1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 80 kDa band observed in human heart and mouse heart lysates (calculated MW of 79.5 kDa band according to NP_004997.4).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ndufs1

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Long Name

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Alternate Names

CI-75kD, Complex I-75kD, EC 7.1.1.2

Entrez Gene IDs

4719 (Human); 227197 (Mouse); 301458 (Rat)

Gene Symbol

NDUFS1

UniProt

NP_004997.4 (Human)

Additional Ndufs1 Products

Product Documents for Ndufs1 Antibody

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Certificate of Analysis

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Product Specific Notices for Ndufs1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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