Ndufs1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-56520

Novus Biologicals, part of Bio-Techne
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NBP1-56520
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Ndufs1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to NDUFS1 (NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)) The peptide sequence was selected from the middle region of NDUFS1)(50ug). Peptide sequence TPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIE The peptide sequence for this immunogen was taken from within the described region.

Specificity

This product is specific to Subunit or Isoform: mitochondrial.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Ndufs1 Antibody

Western Blot: Ndufs1 Antibody [NBP1-56520]

Western Blot: Ndufs1 Antibody [NBP1-56520]

Western Blot: Ndufs1 Antibody [NBP1-56520] - Lanes: Lane1 : 30 ug Human liver Lane2: 30 ug Rat liver Lane3: 30 ug Mouse (wild-type) liver Lane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liver Lane5: 30 ug Human muscle Lane6: 30 ug Rat muscle Lane7: 30 ug Mouse muscle Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Secondary, Antibody Dilution: 1 : 10000 Gene name: NDUFS1.
Western Blot: Ndufs1 Antibody [NBP1-56520]

Western Blot: Ndufs1 Antibody [NBP1-56520]

Western Blot: Ndufs1 Antibody [NBP1-56520] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.
Western Blot: Ndufs1 Antibody [NBP1-56520]

Western Blot: Ndufs1 Antibody [NBP1-56520]

Western Blot: Ndufs1 Antibody [NBP1-56520] - Nuclear lysates at 1:1000.

Applications for Ndufs1 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-56520 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ndufs1

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency.

Long Name

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Alternate Names

CI-75kD, Complex I-75kD, EC 7.1.1.2

Gene Symbol

NDUFS1

UniProt

P28331 (Human)

Additional Ndufs1 Products

Product Documents for Ndufs1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ndufs1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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