NDUFS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52061

Novus Biologicals, part of Bio-Techne
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NBP1-52061
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NDUFS2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SRGVRQWQPDVE corresponding to internal region according to NP_001159631.1.

Epitope

C-SRGVRQWQPDVE

Specificity

This antibody is expected to recognize both reported isoforms (NP_004541.1; NP_001159631.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NDUFS2 Antibody

Western Blot: NDUFS2 Antibody [NBP1-52061]

Western Blot: NDUFS2 Antibody [NBP1-52061]

Western Blot: NDUFS2 Antibody [NBP1-52061] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for NDUFS2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human, mouse and rat heart lysates (calculated MW of 51.9 kDa band according to NP_001159631.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFS2

Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM]

Alternate Names

CI-49, CI-49kD, complex 1, mitochondrial respiratory chain, 49-KD subunit, complex I 49kDa subunit, Complex I-49kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 2 (49kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, NADH-ubiquinone oxidoreductase 49 kDa subunit, NADH-ubiquinone oxidoreductase NDUFS2 subunit

Entrez Gene IDs

4720 (Human); 226646 (Mouse); 289218 (Rat)

Gene Symbol

NDUFS2

UniProt

NP_004541.1 (Human)

Additional NDUFS2 Products

Product Documents for NDUFS2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NDUFS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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