Nebulin Antibody (1F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00004703-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nebulin Antibodies »

Product Specifications

Immunogen

NEB (NP_004534, 2108 a.a. ~ 2217 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NTKTSYHTPADMLSVTAAKDAQANITNTNYKHLIHKYILLPDAMNIELTRNMNRIQSDNEYKQDYNEWYKGLGWSPAGSLEVEKAKKATEYASDQKYRQHPSNFQFKKLT

Specificity

NEB (1F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Nebulin Antibody (1F4)

Sandwich ELISA: Nebulin Antibody (1F4) [H00004703-M01] - Detection limit for recombinant GST tagged NEB is 0.3 ng/ml as a capture antibody.

Applications for Nebulin Antibody (1F4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nebulin

This gene encodes nebulin, a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-,and developmental stage-specific. The 6,669-residue predicted protein, the short form, contains 185 copies of 35-amino acid modules that can be classified into 7 types. It is suggested that alternative splicing may explain the developmental or tissue-specific size variants of nebulin. However, the nucleotide sequence information is not available for other spliced products. Mutations in this gene are associated with recessive nemaline myopathy.

Alternate Names

DKFZp686C1456, FLJ11505, FLJ36536, FLJ39568, FLJ39584, NEB177D, nebulin, NEM2

Entrez Gene IDs

4703 (Human)

Gene Symbol

NEB

OMIM

161650 (Human)

UniProt

NP_004534 (Human)

Additional Nebulin Products

Product Documents for Nebulin Antibody (1F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nebulin Antibody (1F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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