NECAB1 Antibody (CL0576)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52946
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL0576
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LLKETLNQLQSLQNSLECAMETTEEQTRQERQGPAKPEVLSIQWPGKRSSRRVQRHNSFSPNSP
Epitope
SIQWPGKRSS
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for NECAB1 Antibody (CL0576)
Analysis in human cerebral cortex and pancreas tissues using NBP2-52946 antibody. Corresponding NECAB1 RNA-seq data are presented for the same tissues.
Analysis in human cerebral cortex and pancreas tissues using NBP2-52946 antibody. Corresponding NECAB1 RNA-seq data are presented for the same tissues.![Western Blot: NECAB1 Antibody (CL0576) [NBP2-52946]](https://resources.bio-techne.com/images/products/NECAB1-Antibody-CL0576-Western-Blot-NBP2-52946-img0004.jpg "Western Blot: NECAB1 Antibody (CL0576) [NBP2-52946]")
Western Blot: NECAB1 Antibody (CL0576) [NBP2-52946]
Western Blot: NECAB1 Antibody (CL0576) [NBP2-52946] - Lane 1: Marker [kDa], Lane 2: Human cerebral cortex lysate
Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Applications for NECAB1 Antibody (CL0576)
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NECAB1
Alternate Names
EF hand calcium binding protein 1, EFCBP1, EF-hand calcium binding protein 1, EF-hand calcium-binding protein 1, neuronal calcium binding protein, Neuronal calcium-binding protein 1, N-terminal EF-hand calcium binding protein 1, N-terminal EF-hand calcium-binding protein 1, STIP-1, synaptotagmin interacting protein 1
Gene Symbol
NECAB1
Additional NECAB1 Products
Product Documents for NECAB1 Antibody (CL0576)
Product Specific Notices for NECAB1 Antibody (CL0576)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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