NECAB1 Antibody (OTI4G8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72923

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4G8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NECAB1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NECAB1 (NP_071746) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NECAB1 Antibody (OTI4G8) - Azide and BSA Free

Western Blot: NECAB1 Antibody (OTI4G8)Azide and BSA Free [NBP2-72923]

Western Blot: NECAB1 Antibody (OTI4G8)Azide and BSA Free [NBP2-72923]

Western Blot: NECAB1 Antibody (OTI4G8) - Azide and BSA Free [NBP2-72923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
Flow Cytometry: NECAB1 Antibody (OTI4G8) - Azide and BSA Free [NBP2-72923]

Flow Cytometry: NECAB1 Antibody (OTI4G8) - Azide and BSA Free [NBP2-72923]

Flow Cytometry: NECAB1 Antibody (OTI4G8) - Azide and BSA Free [NBP2-72923] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-NECAB1 antibody, and then analyzed by flow cytometry.
Flow Cytometry: NECAB1 Antibody (OTI4G8) - Azide and BSA Free [NBP2-72923]

Flow Cytometry: NECAB1 Antibody (OTI4G8) - Azide and BSA Free [NBP2-72923]

Flow Cytometry: NECAB1 Antibody (OTI4G8) - Azide and BSA Free [NBP2-72923] - Analysis of Hela cells, using anti-NECAB1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for NECAB1 Antibody (OTI4G8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NECAB1

The NECAB1 gene codes for a N-terminal EF-hand calcium-binding protein 1 that in isoform 1 is 351 amino acids long and 40 kDA and in isoform 2 is 100 amino acids long at 11 kDA. NECAB1 has been linked to diseases such as seizures, schizophrenia, alzheimer's disease, motor neuron disease, neuronitis, and neurodegenerative disease.

Alternate Names

EF hand calcium binding protein 1, EFCBP1, EF-hand calcium binding protein 1, EF-hand calcium-binding protein 1, neuronal calcium binding protein, Neuronal calcium-binding protein 1, N-terminal EF-hand calcium binding protein 1, N-terminal EF-hand calcium-binding protein 1, STIP-1, synaptotagmin interacting protein 1

Gene Symbol

NECAB1

Additional NECAB1 Products

Product Documents for NECAB1 Antibody (OTI4G8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NECAB1 Antibody (OTI4G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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