NEDD9/CASL/HEF1 Antibody (1B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00004739-M01

Novus Biologicals, part of Bio-Techne
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H00004739-M01-100ug
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NEDD9/CASL Antibodies »

Product Specifications

Immunogen

NEDD9 (NP_006394, 101 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PRDTIYQVPPSYQNQGIYQVPTGHGTQEQEVYQVPPSVQRSIGGTSGPHVGKKVITPVRTGHGYVYEYPSRYQKDVYDIPPSHTTQGVYDIPPSSAKGPV

Specificity

NEDD9 - neural precursor cell expressed, developmentally down-regulated 9

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NEDD9/CASL/HEF1 Antibody (1B4)

Western Blot: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01]

Western Blot: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01]

Western Blot: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01] - Analysis of NEDD9 expression in transfected 293T cell line by NEDD9 monoclonal antibody (M01), clone 1B4.Lane 1: NEDD9 transfected lysate(92.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01]

Immunocytochemistry/ Immunofluorescence: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01]

Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01] - Analysis of monoclonal antibody to NEDD9 on HeLa cell . Antibody concentration 10 ug/ml.

Applications for NEDD9/CASL/HEF1 Antibody (1B4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NEDD9/CASL

HEF1, also known as Enhancer of filamentation 1, CRKassociated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene. HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1. This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts, it is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells, it is localized predominantly in the cell periphery with particular concentration in lamellipodia, but it is also found in the nucleus. HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue. HEF1 is also detected in T-cells, B-cells and diverse cell lines. HEF1 is activated upon induction of cell growth. Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9

Alternate Names

CAS2, CASL, HEF1, p105

Entrez Gene IDs

4739 (Human)

Gene Symbol

NEDD9

OMIM

602265 (Human)

UniProt

NP_006394 (Human)

Additional NEDD9/CASL Products

Product Documents for NEDD9/CASL/HEF1 Antibody (1B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NEDD9/CASL/HEF1 Antibody (1B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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