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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4H5

Concentration

0.48 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human NEK11 (NP_079076) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK11 Antibody (OTI4H5)

Western Blot: NEK11 Antibody (OTI4H5) [NBP2-02911]

Western Blot: NEK11 Antibody (OTI4H5) [NBP2-02911]

Western Blot: NEK11 Antibody (4H5) [NBP2-02911] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11.
Immunocytochemistry/Immunofluorescence: NEK11 Antibody (4H5) [NBP2-02911] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK11.

Applications for NEK11 Antibody (OTI4H5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.48 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK11

This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.

Alternate Names

EC 2.7.11, EC 2.7.11.1, FLJ23495, Never in mitosis A-related kinase 11, NIMA (never in mitosis gene a)- related kinase 11, NimA-related protein kinase 11, serine/threonine-protein kinase Nek11

Entrez Gene IDs

79858 (Human)

Gene Symbol

NEK11

Additional NEK11 Products

Product Documents for NEK11 Antibody (OTI4H5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NEK11 Antibody (OTI4H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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