Neogenin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89651

Novus Biologicals, part of Bio-Techne
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NBP1-89651
NBP1-89651-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Neogenin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGR

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 30333477)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neogenin Antibody

Western Blot: Neogenin Antibody [NBP1-89651]

Western Blot: Neogenin Antibody [NBP1-89651]

Western Blot: Neogenin Antibody [NBP1-89651] - Analysis in human cell lines A-549 and HeLa using anti-NEO1 antibody. Corresponding NEO1 RNA-seq data are presented for the same cell lines. Loading control: anti-PARP1.
Immunocytochemistry/ Immunofluorescence: Neogenin Antibody [NBP1-89651]

Immunocytochemistry/ Immunofluorescence: Neogenin Antibody [NBP1-89651]

Immunocytochemistry/Immunofluorescence: Neogenin Antibody [NBP1-89651] - Staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane. Antibody staining shown in green.
Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651]

Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651]

Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
View 3 more images

Applications for Neogenin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Frozen

Reported in scientific literature (PMID: 30333477)

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neogenin

Neogenin is one of several netrin-binding proteins. Neogenin functions as an Repulsive Guidance Molecule (RGM) receptor; RGM is implicated in axonal guidance and neural tube closure.

Alternate Names

NEO, NEO1, NGN

Gene Symbol

NEO1

Additional Neogenin Products

Product Documents for Neogenin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Neogenin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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