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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1411 to 1461 of human Neogenin using the numbering given in entry NP_002490.2 (GeneID 4756).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neogenin Antibody - BSA Free

Western Blot: Neogenin Antibody [NBP2-32272]

Western Blot: Neogenin Antibody [NBP2-32272]

Western Blot: Neogenin Antibody [NBP2-32272] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody NBP2-32272 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Neogenin Antibody [NBP2-32272]

Immunoprecipitation: Neogenin Antibody [NBP2-32272]

Immunoprecipitation: Neogenin Antibody [NBP2-32272] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody NBP2-32272 used for IP at 6 ug per reaction. Neogenin was also immunoprecipitated by rabbit anti-Neogenin antibody BL16480. For blotting immunoprecipitated Neogenin, NBP2-32272 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Neogenin Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Neogenin

Neogenin is one of several netrin-binding proteins. Neogenin functions as an Repulsive Guidance Molecule (RGM) receptor; RGM is implicated in axonal guidance and neural tube closure.

Alternate Names

NEO, NEO1, NGN

Gene Symbol

NEO1

Additional Neogenin Products

Product Documents for Neogenin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neogenin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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