Neomycin phosphotransferase II Antibody (4B4D1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60487

Novus Biologicals, part of Bio-Techne
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NB110-60487
NB110-60487-0.025ml
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Key Product Details

Species Reactivity

Validated:

Bacteria, Non-species specific

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4B4D1

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Neomycin phosphotransferase II Antibodies »

Product Summary for Neomycin phosphotransferase II Antibody (4B4D1) - BSA Free

Immunogen

Purified recombinant fragment of Neomycin phosphotransferase II expressed in E. Coli.

Reactivity Notes

Transfected or transformed cells / organisms (plants, animals) carrying NPTII gene encoding neomycin phosphotransferase II enzyme. Escherichia coli (E. coli).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Neomycin phosphotransferase II Antibody (4B4D1) - BSA Free

Western Blot: Neomycin phosphotransferase II Antibody (4B4D1)BSA Free [NB110-60487]

Western Blot: Neomycin phosphotransferase II Antibody (4B4D1)BSA Free [NB110-60487]

Western Blot: Neomycin phosphotransferase II Antibody (4B4D1) [NB110-60487] - Figure 1: Western blot analysis using anti-human NPT monoclonal antibody against recombinant NPT expressed in E. Coli.

Applications for Neomycin phosphotransferase II Antibody (4B4D1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neomycin phosphotransferase II

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Alternate Names

NPT2, NPTII

Additional Neomycin phosphotransferase II Products

Product Documents for Neomycin phosphotransferase II Antibody (4B4D1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Neomycin phosphotransferase II Antibody (4B4D1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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