Nephrin Antibody (Y17-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30130

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-30130-0.05ml
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # Y17-R

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nephrin Antibodies »

Product Specifications

Immunogen

Peptide derived from the center of the fifth Ig-like domain of human Nephrin. Antibody recognizes the epitope located between Cys465 - Cys528.

Epitope

Cys465 - Cys528

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for Nephrin Antibody (Y17-R)

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] - Stiffness-mediated differentiation phenotype induces upregulation of podocyte-specific functional proteins. Representative image of Western blot for Nephrin antibody critical for physiological function of podocytes. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep43934) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130]

Immunocytochemistry/ Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130]

Immunocytochemistry/Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130] - Nephrin expression in HEK293 cells, visualized with clonal rabbit antiNephrin monospecific antibody. Primary antibody dilution - 1:70
Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Western Blot: Nephrin Antibody (I) [NBP1-30130] - Analysis of mouse nephrin: lane 1 - 50ng; lane 2 - 100ng; lane 3 -200ng of recombinant mouse nephrin.
View 1 more images

Applications for Nephrin Antibody (Y17-R)

Application
Recommended Usage

ELISA

1:50000-1:100000

Immunoprecipitation

1:10-1:500

Western Blot

1:5000
Application Notes
Use in IHC-P reported in scientific literature (PMID:34516901). For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS). ; 0.1% Triton X-100. Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milk. Use in Immunohistochemistry reported in scientific literature (PMID: 25129444). .

Reviewed Applications

Read 1 review rated 4 using NBP1-30130 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nephrin

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Alternate Names

CNF, NPHS1

Entrez Gene IDs

4868 (Human); 54631 (Mouse); 64563 (Rat)

Gene Symbol

NPHS1

UniProt

Q9QZS7, O60500, Q9R044 (Rat)

Additional Nephrin Products

Product Documents for Nephrin Antibody (Y17-R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nephrin Antibody (Y17-R)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.