Neprilysin/CD10 Antibody (5E9A5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37301

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5E9A5

Format

BSA Free

Concentration

1 mg/ml

View all Neprilysin/CD10 Antibodies »

Product Summary for Neprilysin/CD10 Antibody (5E9A5) - BSA Free

Immunogen

Purified recombinant fragment of human Neprilysin/CD10 (AA: 52-246) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

85.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neprilysin/CD10 Antibody (5E9A5) - BSA Free

Western Blot: Neprilysin/CD10 Antibody (5E9A5)BSA Free [NBP2-37301]

Western Blot: Neprilysin/CD10 Antibody (5E9A5)BSA Free [NBP2-37301]

Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Neprilysin/CD10 Antibody (5E9A5) - BSA Free [NBP2-37301]

Immunohistochemistry: Neprilysin/CD10 Antibody (5E9A5) - BSA Free [NBP2-37301]

Immunohistochemistry: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD10 mouse mAb with DAB staining.
Western Blot: Neprilysin/CD10 Antibody (5E9A5)BSA Free [NBP2-37301]

Western Blot: Neprilysin/CD10 Antibody (5E9A5)BSA Free [NBP2-37301]

Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against human CD10 (AA: 52-246) recombinant protein. (Expected MW is 41.4 kDa)
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Applications for Neprilysin/CD10 Antibody (5E9A5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neprilysin/CD10

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Gene Symbol

MME

Additional Neprilysin/CD10 Products

Product Documents for Neprilysin/CD10 Antibody (5E9A5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Neprilysin/CD10 Antibody (5E9A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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