Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44364

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL12448

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Neprilysin/CD10 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P08473, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free

Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP3-44364]

Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP3-44364]

Analysis in human small intestine and pancreas tissues using NBP3-44364 antibody. Corresponding Neprilysin/CD10 RNA-seq data are presented for the same tissues.
Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP3-44364]

Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP3-44364]

Staining of human kidney shows strong apical membranous positivity in cells in tubules and cells in glomeruli.
Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP3-44364]

Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP3-44364]

Staining of human pancreas shows very weak positivity in exocrine glandular cells.
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Applications for Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500-1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neprilysin/CD10

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Gene Symbol

MME

Additional Neprilysin/CD10 Products

Product Documents for Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Neprilysin/CD10 Antibody (CL12448) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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