Nesfatin-1/Nucleobindin-2 Antibody (6H4)
Novus Biologicals, part of Bio-Techne | Catalog # H00004925-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 6H4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NUCB2 (NP_005004.1, 322 a.a. ~ 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEKKEFLEPDSWETLDQQQFFTEEELKEYENIIALQENELKKKADELQKQKEELQRQHDQLEAQKLEYHQVIQQMEQKKLQQGIPPSGPAGELKFEPHI
Specificity
This product is specific for Human NUCB2 monoclonal antibody (M03), clone 6H4 [Gene ID: 4925].
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Nesfatin-1/Nucleobindin-2 Antibody (6H4)
![Western Blot: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03]](https://resources.bio-techne.com/images/products/Nesfatin-1-Nucleobindin-2-Antibody-6H4-Western-Blot-H00004925-M03-img0003.jpg "Western Blot: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03]")
Western Blot: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03]
Western Blot: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03] - Analysis of NUCB2 expression in HepG2.![Immunocytochemistry/ Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03]](https://resources.bio-techne.com/images/products/Nesfatin-1-Nucleobindin-2-Antibody-6H4-Immunocytochemistry-Immunofluorescence-H00004925-M03-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03]")
Immunocytochemistry/ Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03]
Immunocytochemistry/Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03] - Analysis of monoclonal antibody to NUCB2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03] - Detection limit for recombinant GST tagged NUCB2 is 0.3 ng/ml as a capture antibody.
Applications for Nesfatin-1/Nucleobindin-2 Antibody (6H4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nesfatin-1/Nucleobindin-2
Alternate Names
NEFA, Nesfatin1, NUCB2, Nucleobindin-2
Gene Symbol
NUCB2
UniProt
Additional Nesfatin-1/Nucleobindin-2 Products
Product Documents for Nesfatin-1/Nucleobindin-2 Antibody (6H4)
Product Specific Notices for Nesfatin-1/Nucleobindin-2 Antibody (6H4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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