Nesfatin-1/Nucleobindin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87383

Novus Biologicals, part of Bio-Techne
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NBP1-87383
NBP1-87383-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nesfatin-1/Nucleobindin-2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSEVDTNKDRLVTLEEFLKATEKKEFLEPDSWETLDQQQFFTEEELKEYENIIALQENELKKKADELQKQKEELQRQHDQLEAQKLEYHQVIQQMEQKKLQQGIPPSGPAGELKFEPR

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31670281). Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nesfatin-1/Nucleobindin-2 Antibody

Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383]

Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383]

Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383]

Immunocytochemistry/ Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383]

Immunocytochemistry/Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383]

Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383]

Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
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Applications for Nesfatin-1/Nucleobindin-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nesfatin-1/Nucleobindin-2

Nucleobindin-2 is a calcium-binding EF-hand protein.[supplied by OMIM]

Alternate Names

NEFA, Nesfatin1, NUCB2, Nucleobindin-2

Gene Symbol

NUCB2

Additional Nesfatin-1/Nucleobindin-2 Products

Product Documents for Nesfatin-1/Nucleobindin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nesfatin-1/Nucleobindin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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