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Nesfatin-1/Nucleobindin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12972

Novus Biologicals, part of Bio-Techne
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NBP3-12972

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nesfatin-1/Nucleobindin-2. The exact sequence is proprietary.

Reactivity Notes

Mouse (84%), Rat (87%), Chicken (80%), Bovine (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Nesfatin-1/Nucleobindin-2 Antibody

Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972]

Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972]

Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972]

Immunocytochemistry/ Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972]

Immunocytochemistry/Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nesfatin-1/Nucleobindin-2 protein stained by Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972]

Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972]

Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Nesfatin-1/Nucleobindin-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nesfatin-1/Nucleobindin-2

Nucleobindin-2 is a calcium-binding EF-hand protein.[supplied by OMIM]

Alternate Names

NEFA, Nesfatin1, NUCB2, Nucleobindin-2

Gene Symbol

NUCB2

Additional Nesfatin-1/Nucleobindin-2 Products

Product Documents for Nesfatin-1/Nucleobindin-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nesfatin-1/Nucleobindin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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