Nesprin 2 Antibody (10H8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59945
![Western Blot: Nesprin 2 Antibody (10H8)BSA Free [NBP2-59945]](https://resources.bio-techne.com/images/products/Nesprin-2-Antibody-10H8-Western-Blot-NBP2-59945-img0001.jpg "Western Blot: Nesprin 2 Antibody (10H8)BSA Free [NBP2-59945]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 10H8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
GST fused to residues 6,653-6,835 of human Nesprin 2
Specificity
Specificity of human Nesprin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Nesprin 2 Antibody (10H8) - BSA Free
Western Blot: Nesprin 2 Antibody (10H8)BSA Free [NBP2-59945]
Western Blot: Nesprin 2 Antibody (10H8) [NBP2-59945] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)![Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody (10H8) - BSA Free [NBP2-59945]](https://resources.bio-techne.com/images/products/Nesprin-2-Antibody-10H8-Immunocytochemistry-Immunofluorescence-NBP2-59945-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody (10H8) - BSA Free [NBP2-59945]")
Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody (10H8) - BSA Free [NBP2-59945]
Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (10H8) [NBP2-59945] - Staining in HeLa cells labeled with either 6C11 or 10H8 monoclonal antibodies raised against human Nesp2. The antibodies can be used on either formaldehyde- or methanol-fixed cells.Applications for Nesprin 2 Antibody (10H8) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1-2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nesprin 2
Alternate Names
DKFZp434H2235, DKFZp686E01115, DKFZp686H1931, EDMD5, FLJ46790, KIAA1011FLJ11014, nesprin 2, Nesprin-2, NUAFLJ43727, NUANCEFLJ45710, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, polytrophin, Protein NUANCE, spectrin repeat containing, nuclear envelope 2, Synaptic nuclear envelope protein 2, synaptic nuclei expressed gene 2, Syne-2, TROPH
Gene Symbol
SYNE2
Additional Nesprin 2 Products
Product Documents for Nesprin 2 Antibody (10H8) - BSA Free
Product Specific Notices for Nesprin 2 Antibody (10H8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...