Nestin Antibody (4D11) [Janelia Fluor® 549]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92717JF549

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for Nestin Antibody (4D11) [Janelia Fluor® 549]

Immunogen

Recombinant construct, amino acids 317-630 of the human Nestin protein expressed in and purified from E. coli. [UniProt P48681]

Marker

Neural Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Nestin Antibody (4D11) [Janelia Fluor® 549]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Alternate Names

NES

Gene Symbol

NES

Additional Nestin Products

Product Documents for Nestin Antibody (4D11) [Janelia Fluor® 549]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nestin Antibody (4D11) [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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