Nestin Antibody (CL0197) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44499

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0197

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Nestin Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P48681, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nestin Antibody (CL0197) - Azide and BSA Free

Nestin Antibody (CL0197) - Azide and BSA Free Western Blot: Nestin Antibody [NBP3-44499]

Western Blot: Nestin Antibody [NBP3-44499]

Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
Nestin Antibody (CL0197) - Azide and BSA Free Immunohistochemistry-Paraffin: Nestin Antibody [NBP3-44499]

Immunohistochemistry-Paraffin: Nestin Antibody [NBP3-44499]

Staining of human kidney shows strong positivity in renal glomeruli.
Nestin Antibody (CL0197) - Azide and BSA Free Immunohistochemistry-Paraffin: Nestin Antibody [NBP3-44499]

Immunohistochemistry-Paraffin: Nestin Antibody [NBP3-44499]

Staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.
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Applications for Nestin Antibody (CL0197) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Alternate Names

NES

Gene Symbol

NES

Additional Nestin Products

Product Documents for Nestin Antibody (CL0197) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nestin Antibody (CL0197) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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