Nestin Antibody (CL0197) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44499
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL0197
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P48681, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Nestin Antibody (CL0197) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
: 2-10 ug/ml
Immunohistochemistry
: 1:2500 - 1:5000
Immunohistochemistry-Paraffin
: 1:2500 - 1:5000
Western Blot
: 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Lyophilized in 5% Trehalose.
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nestin
Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.
Alternate Names
NES
Gene Symbol
NES
Additional Nestin Products
Product Documents for Nestin Antibody (CL0197) - Azide and BSA Free
Product Specific Notices for Nestin Antibody (CL0197) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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