NeuroD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13350
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (94%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for NeuroD1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of mouse NeuroD1. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for NeuroD1 Antibody
![Immunocytochemistry/ Immunofluorescence: NeuroD1 Antibody [NBP3-13350]](https://resources.bio-techne.com/images/products/NeuroD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13350-img0002.jpg "Immunocytochemistry/ Immunofluorescence: NeuroD1 Antibody [NBP3-13350]")
Immunocytochemistry/ Immunofluorescence: NeuroD1 Antibody [NBP3-13350]
Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody [NBP3-13350] - NeuroD1 antibody detects NeuroD1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NeuroD1 stained by NeuroD1 antibody (NBP3-13350) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP3-13350]](https://resources.bio-techne.com/images/products/NeuroD1-Antibody-Immunohistochemistry-Paraffin-NBP3-13350-img0001.jpg "Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP3-13350]")
Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP3-13350]
Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP3-13350] - E10.5 mouse embryo. NeuroD1 stained by NeuroD1 antibody (NBP3-13350) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: NeuroD1 Antibody [NBP3-13350] -
Western Blot: NeuroD1 Antibody [NBP3-13350] -IMR32 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuroD1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.Applications for NeuroD1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NeuroD1
Long Name
Neurogenic Differentiation Factor 1
Alternate Names
BETA2, BHF-1, NIDDM
Gene Symbol
NEUROD1
Additional NeuroD1 Products
Product Specific Notices for NeuroD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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