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Neurofascin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13182

Novus Biologicals, part of Bio-Techne
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NBP3-13182

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Neurofascin. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Neurofascin Antibody

Western Blot: Neurofascin Antibody [NBP3-13182]

Western Blot: Neurofascin Antibody [NBP3-13182]

Western Blot: Neurofascin Antibody [NBP3-13182] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (NBP3-13182) diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: Neurofascin Antibody [NBP3-13182]

Immunocytochemistry/ Immunofluorescence: Neurofascin Antibody [NBP3-13182]

Immunocytochemistry/Immunofluorescence: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182]

Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182]

Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for Neurofascin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neurofascin

NFASC (Neurofascin) is involved in cell adhesion, axon subcellular targeting, synapse formation, regulating functions of the brain, and axonal guidance. NFASC is known to have interactions with SDCBP, ABL1, ANK1, CRK and DCX. NFASC has been studied in relation to multiple sclerosis, carcinoma, neuronitis and Parkinson's disease.

Alternate Names

NFASC, NRCAML

Gene Symbol

NFASC

Additional Neurofascin Products

Product Documents for Neurofascin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neurofascin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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