Neurofascin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13182
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Human (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Neurofascin. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Neurofascin Antibody
Western Blot: Neurofascin Antibody [NBP3-13182]
Western Blot: Neurofascin Antibody [NBP3-13182] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (NBP3-13182) diluted at 1:2000.Immunocytochemistry/ Immunofluorescence: Neurofascin Antibody [NBP3-13182]
Immunocytochemistry/Immunofluorescence: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182]
Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.Applications for Neurofascin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neurofascin
Alternate Names
NFASC, NRCAML
Gene Symbol
NFASC
Additional Neurofascin Products
Product Specific Notices for Neurofascin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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