Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # McNFn27a

Format

BSA Free

Concentration

1 mg/ml

View all Neurofibromin 1 Antibodies »

Product Specifications

Immunogen

A highly conserved N-terminal peptide, corresponding to amino acids 27-41 of human Neurofibromin 1. [UniProt# P21359]

Localization

Cytoplasmic

Specificity

This is specific for full-length or truncated forms of neurofibromin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Neurofibromin 1 Antibody (McNFn27a) - BSA Free

Western Blot: Neurofibromin 1 Antibody (McNFn27a)BSA Free [NB300-153]

Western Blot: Neurofibromin 1 Antibody (McNFn27a)BSA Free [NB300-153]

Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.
Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) - BSA Free [NB300-153]

Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) - BSA Free [NB300-153]

Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.

Applications for Neurofibromin 1 Antibody (McNFn27a) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50. Use reported in scientific literature

Immunoprecipitation

1:10-1:500

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neurofibromin 1

Neurofibromin 1 (NF1), the Nf1 gene product, is a large protein which contains a central Ras-GTPase-activating (RasGAP) domain and is suggested to function as a negative regulator of proto-oncogene RAS and downstream effectors. It stimulates the GTPase activity of RAS and acts as regulator of RAS signaling. Consistent with its biochemical activity, NF1 functions as a tumor-suppressor gene in juvenile myelomonocytic leukemia (JMML), and diseased BM cells exhibit aberrant Raf/MEK/ERK signaling. Moreover, NF1 shows greater affinity for Ras GAP, but lower specific activity. Besides JMML, defects in NF1 have also been linked to neurofibromatosis type 1 (NF1), Watson syndrome (WS), familial spinal neurofibromatosis (FSNF), neurofibromatosis-Noonan syndrome (NFNS), colorectal cancer (CRC).

Alternate Names

DKFZp686J1293, FLJ21220, Neurofibromatosis-related protein NF-1, neurofibromin, neurofibromin 1, NFNS, VRNF, WSS

Gene Symbol

NF1

Additional Neurofibromin 1 Products

Product Documents for Neurofibromin 1 Antibody (McNFn27a) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Neurofibromin 1 Antibody (McNFn27a) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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