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Neurogenin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68155

Novus Biologicals, part of Bio-Techne
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NB100-68155

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence KDLLHTTPCFIPYH corresponding to C-Terminus according to NP_006152.2.

Epitope

KDLLHTTPCFIPYH

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Applications for Neurogenin-1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000
Application Notes
WB: Preliminary experiments gave an approx. 37 kDa band in nuclear lysates of HeLa after 2ug/ml antibody staining. The 37 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neurogenin-1

Neurogenin 1 is the protein product of an activator-type bHLH gene (Ngn1). The activator-type bHLH genes (which also include Mash1 and Math) promote neuronal fate determination and induce expression of Notch ligands such as Delta which activate Notch signaling. The activator type bHLH genes are antagonized by repressor-type basic helix-loop-helix (bHLH) genes such as Hes1, 3 and 5. Thus, activator-type and repressor-type bHLH genes regulate each other, allowing only subsets of neural stem cells to undergo differentiation. Neurogenin 1 is thus one of the key regulators of neural stem cell differentiation, which is essential for generation of complex brain structures of appropriate size, shape and cell arrangement.

Alternate Names

AKA, Math4C, NEUROD3, NEUROG1, Neurogenin1

Entrez Gene IDs

4762 (Human); 18014 (Mouse); 29410 (Rat)

Gene Symbol

NEUROG1

Additional Neurogenin-1 Products

Product Documents for Neurogenin-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neurogenin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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