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Neurogenin-3 Antibody (PCRP-NEUROG3-1E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13987

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-13987-100ug
NBP3-13987-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-NEUROG3-1E10

Concentration

0.2 mg/ml

Product Summary for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10)

Immunogen

Recombinant human full-lengthNeurogenin-3protein (Uniprot: Q9Y4Z2)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14029)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10)

Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987]

Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987]

Immunocytochemistry/Immunofluorescence: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987]

Flow Cytometry: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987]

Flow Cytometry: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987] - Flow cytometric analysis of PFA-fixed HeLa cells. Neurogenin-3 antibody (PCRP-NEUROG3-1E10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987]

Protein Array: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987]

Protein Array: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) [NBP3-13987] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Neurogenin-3 Antibody (PCRP-NEUROG3-1E10).

Applications for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Neurogenin-3

Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm (Gradwohl et al., 2000 [PubMed 10677506]).[supplied by OMIM]

Alternate Names

Atoh5, Math4B, NEUROG3, Neurogenin3, Relax

Gene Symbol

NEUROG3

UniProt

Additional Neurogenin-3 Products

Product Documents for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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