Neurokinin B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92178
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Neurokinin B Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GAVCKEPQEEVVPGGGRSKRDPDLYQLLQRLFKSHSSLEGLLKALSQASTDPKESTSPEKRDMHDFFVGLM
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Neurokinin B Antibody
![Western Blot: Neurokinin B Antibody [NBP1-92178]](https://resources.bio-techne.com/images/products/Neurokinin-B-Antibody-Western-Blot-NBP1-92178-img0005.jpg "Western Blot: Neurokinin B Antibody [NBP1-92178]")
Western Blot: Neurokinin B Antibody [NBP1-92178]
Western Blot: Neurokinin B Antibody [NBP1-92178] - Analysis in control (vector only transfected HEK293T lysate) and TAC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody [NBP1-92178]](https://resources.bio-techne.com/images/products/Neurokinin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92178-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody [NBP1-92178]")
Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody [NBP1-92178]
Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] - Staining of human cell line U-2 OS shows localization to vesicles.![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178]](https://resources.bio-techne.com/images/products/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-92178-img0002.jpg "Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178]")
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178]
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.Applications for Neurokinin B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neurokinin B
Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).
Alternate Names
gamma tachykinin 3, neurokinin beta, neurokinin B-like, neuromedin K, preprotachykinin B, PRO1155, tachykinin 3, tachykinin-3, ZNEUROK1NKNBNKB
Gene Symbol
TAC3
Additional Neurokinin B Products
Product Documents for Neurokinin B Antibody
Product Specific Notices for Neurokinin B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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