Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S97A-31

Concentration

1 mg/ml

View all Neuroligin 1/NLGN1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3.

Localization

Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density

Specificity

Greater then 40% identity with Neuroligin-2 and -3.Detects approx 120kDa. Does not cross-react with other Neuroligins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Neuroligin 1/NLGN1 Antibody (S97A-31)

Western Blot: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192]

Western Blot: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192]

Western Blot: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Western Blot analysis of Mouse Brain Membrane showing detection of ~120 kDa Neuroligin 1/NLGN1 protein using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~120 kDa.
Immunocytochemistry/ Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192]

Immunocytochemistry/ Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192]

Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1/NLGN1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192]

Immunocytochemistry/ Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192]

Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.
View 1 more images

Applications for Neuroligin 1/NLGN1 Antibody (S97A-31)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
2 ug/ml of Neuroligin 1 Antibody was sufficient for detection of Neuroligin-1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neuroligin 1/NLGN1

Neuroligin 1 encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

Alternate Names

Nlg, NLGN1

Gene Symbol

NLGN1

UniProt

Q62765

Additional Neuroligin 1/NLGN1 Products

Product Documents for Neuroligin 1/NLGN1 Antibody (S97A-31)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neuroligin 1/NLGN1 Antibody (S97A-31)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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