Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S110-29

Concentration

1 mg/ml

View all Neuroligin 3/NLGN3 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human and Mouse.

Localization

Cell Membrane , Cell Junction , Synapse

Specificity

Approx 60% identity with Neuroligin-1. approx 40% identity with Neuroligin-2.Detects approx 110kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Neuroligin 3/NLGN3 Antibody (S110-29)

Western Blot: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200]

Western Blot: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200]

Western Blot: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3/NLGN3 protein using Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody, Clone S110-29 (NBP2-42200). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody (NBP2-42200) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.
Immunocytochemistry/ Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200]

Immunocytochemistry/ Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200]

Immunocytochemistry/Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody, Clone S110-29 (NBP2-42200). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody (NBP2-42200) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 3/NLGN3 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200]

Immunocytochemistry/ Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200]

Immunocytochemistry/Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse . Magnification: 60X.
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Applications for Neuroligin 3/NLGN3 Antibody (S110-29)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of Neuroligin 3 Antibody was sufficient for detection of Neuroligin 3 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neuroligin 3/NLGN3

Neuroligin 3 encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but their full length sequences have not been determined.

Alternate Names

ASPGX1, AUTSX1, HNL3, NL3, NLGN3

Gene Symbol

NLGN3

UniProt

Q62889 (Human)

Additional Neuroligin 3/NLGN3 Products

Product Documents for Neuroligin 3/NLGN3 Antibody (S110-29)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neuroligin 3/NLGN3 Antibody (S110-29)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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