Neuroligin 3/NLGN3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79866

Novus Biologicals, part of Bio-Techne
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NB100-79866
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Neuroligin 3/NLGN3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RKDKRRQEPLRQPS corresponding to internal region according to NP_061850.2.

Epitope

RKDKRRQEPLRQPS

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Neuroligin 3/NLGN3 Antibody

Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866]

Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866]

Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866] - HEK293 overexpressing Neuregulin3 and probed with (mock transfection in first lane).
Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866]

Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866]

Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866] - (0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Neuroligin 3/NLGN3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.05 - 0.2 ug/ml
Application Notes
WB: Approx. 85-90 kDa band observed in human brain (amygdala) lysates (calculated MW of 91.6 kDa band according to NP_061850.2). In transfected HEK293 transiently expressing Neuregulin3 a band of approx. 98 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neuroligin 3/NLGN3

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but their full length sequences have not been determined.

Alternate Names

ASPGX1, AUTSX1, HNL3, NL3, NLGN3

Entrez Gene IDs

54413 (Human); 245537 (Mouse); 171297 (Rat)

Gene Symbol

NLGN3

UniProt

NP_061850.2 (Human)

Additional Neuroligin 3/NLGN3 Products

Product Documents for Neuroligin 3/NLGN3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Neuroligin 3/NLGN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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