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Neuroligin 3/NLGN3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12987

Novus Biologicals, part of Bio-Techne
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NBP3-12987

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Neuroligin 3/NLGN3. The exact sequence is proprietary.

Reactivity Notes

Japanese Medaka (82%), Xenopus laevis (83%), Chicken (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Neuroligin 3/NLGN3 Antibody

Western Blot: Neuroligin 3/NLGN3 Antibody [NBP3-12987]

Western Blot: Neuroligin 3/NLGN3 Antibody [NBP3-12987]

Western Blot: Neuroligin 3/NLGN3 Antibody [NBP3-12987] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuroligin 3/NLGN3 antibody (NBP3-12987) diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Neuroligin 3/NLGN3 Antibody [NBP3-12987]

Immunocytochemistry/ Immunofluorescence: Neuroligin 3/NLGN3 Antibody [NBP3-12987]

Immunocytochemistry/Immunofluorescence: Neuroligin 3/NLGN3 Antibody [NBP3-12987] - Neuroligin 3/NLGN3 antibody detects Neuroligin 3/NLGN3 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuroligin 3/NLGN3 protein stained by Neuroligin 3/NLGN3 antibody (NBP3-12987) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP3-12987]

Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP3-12987]

Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP3-12987] - Rat brain. Neuroligin 3/NLGN3 antibody (NBP3-12987) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for Neuroligin 3/NLGN3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neuroligin 3/NLGN3

Neuroligin 3 encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but their full length sequences have not been determined.

Alternate Names

ASPGX1, AUTSX1, HNL3, NL3, NLGN3

Gene Symbol

NLGN3

Additional Neuroligin 3/NLGN3 Products

Product Documents for Neuroligin 3/NLGN3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neuroligin 3/NLGN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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