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Neuron-specific beta-III Tubulin Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1195G

R&D Systems, part of Bio-Techne
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IC1195G-100

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Avian - Coturnix japonica (Quail)

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunocytochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1

Product Specifications

Immunogen

Rat brain-derived microtubules

Specificity

Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images

Detection of beta-III Tubulin in A172 cells by Flow Cytometry

A172 cells were stained with Mouse Anti-Neuron-specific beta-III Tubulin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1195G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

Applications

Application
Recommended Usage

Intracellular Staining by Flow Cytometry


Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta-III Tubulin, also known as tubulin beta-4, is regarded as a neuron-specific marker. The expression of beta-III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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