Neuron-specific beta-III Tubulin Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1195G
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Avian - Coturnix japonica (Quail)
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Immunocytochemistry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Product Specifications
Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images
Detection of beta-III Tubulin in HepG2 cells by Flow Cytometry.
HepG2 cells were stained with Mouse Anti-Neuron-specific beta-III Tubulin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1195G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment was HepG2 cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and
Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-III Tubulin
Alternate Names
beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III
Entrez Gene IDs
10381 (Human)
Gene Symbol
TUBB3
Additional beta-III Tubulin Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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