Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

beta-III Tubulin in Rat Cortical Stem Cells.

beta‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1195R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse beta-III Tubulin by Immunocytochemistry/Immunofluorescence

Assessment of the roles of macrophages in corneal development. a Co-staining of limbal blood vessels/corneal nerve fibers and macrophages in mice at postnatal day 7 (P7). The upper image shows the immunostaining of the limbal blood vessels and macrophages with anti-CD31 antibody and anti-CD64 antibody (scale bar, 10 μm). The lower image shows the immunostaining of the corneal nerve fibers and macrophages with anti-beta -III-tubulin antibody and anti-CD64 antibody (scale bar, 10 μm). b The gating strategies of analyzing corneal macrophages with flow cytometry. Corneal macrophages in mice at P7 were classified into C–C chemokine receptor type 2 negative (CCR2−) and positive (CCR2+) populations. The gating strategies were justified by the related isotype controls in Supplementary Fig. 6. c The expression of genes involved in cell division (left image) and neurogenesis and angiogenesis (right image) in the flow-cytometry-sorted corneal CCR2− and CCR2+ macrophages with qPCR (n = 3 independent experiments, ten mice per experiment in each group). d Changes in the corneal development of anti-CSF1R antibody-treated mice (n = 6 mice at each time point in each group). e Alteration of the expression of genes involved in cell division, neurogenesis, and angiogenesis in corneal tissues of anti-CSF1R antibody-treated mice at P28 (n = 4 independent experiments, six mice per experiment in each group). f Changes in the corneal development of BMS CCR2 22-treated mice (n = 6 mice at each time point in each group). The data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31434991), licensed under a CC-BY license. Not internally tested by R&D Systems.