Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A))
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64392
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # ELA10-101.5 (39A)
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Elastase from human neutrophils
Reactivity Notes
Not yet tested in other species.
Localization
Secreted
Specificity
NB100-64392 is specific for human neutrophil elastase.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A))
Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) - BSA Free [NB100-64392]
Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.Applications for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A))
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
<0.1% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neutrophil Elastase/ELA2
Alternate Names
ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin
Entrez Gene IDs
1991 (Human)
Gene Symbol
ELANE
UniProt
Additional Neutrophil Elastase/ELA2 Products
Product Documents for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A))
Product Specific Notices for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A))
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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