NF-H Antibody (9B12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05210

Novus Biologicals, part of Bio-Techne
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NBP1-05210
NBP1-05210-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 9B12

Concentration

1 mg/ml

View all NF-H Antibodies »

Product Specifications

Immunogen

Native NF-H purified from bovine spinal cord, binding to phosphorylated KSP sequences.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-H Antibody (9B12)

Western Blot: NF-H Antibody (9B12) [NBP1-05210]

Western Blot: NF-H Antibody (9B12) [NBP1-05210]

Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.
Immunocytochemistry/ Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210]

Immunocytochemistry/ Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210]

Immunocytochemistry/Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210] - Mixed neuron/glial cultures stained with NBP1-05210 (red) and rabbit GFAP antibody NB300-141 (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).
Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210]

Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210]

Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210] - Analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, dilution 1:5,000 (Green). Hoechst staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibody. The antibody is a robust marker of the axons of neuronal cells.

Applications for NF-H Antibody (9B12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunohistochemistry Free-Floating

1:1000

Western Blot

1:10000
Application Notes
This 200kDa Neurofilament Heavy antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-H

Neuronal intermediate filaments (NIF) are found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 200kD neurofilament heavy (NEFH) is one of the major components of neuronal cytoskeleton NIF and NEFH gene was originally proposed as a DNA marker for presymptomatic diagnosis in neurofibromatosis type 2 (NF2) . NEHF facilitate interactions between NIF and brain mitochondria with binding efficiency dependent upon mitochondrial membranes potential. NEFH and beta-catenin are constituents in postsynaptic density (PSD) in CNS and NEFH is essential for maintaining normal cell integrity. Diminished NEFH levels are seen in certain cancers including human autonomic nerve tumors or central neurocytomas, prostate cancer, clear-cell epithelioid tumor and small cell lung carcinoma. Fibroblasts overexpressing NEFH are distorted, multinucleated, and possess inclusions, typical changes seen in neuropathies, and defects in NEFH are linked to susceptibility to ALS.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

24587 (Rat)

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for NF-H Antibody (9B12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NF-H Antibody (9B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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