NF-H Antibody (AH1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05209
![Western Blot: NF-H Antibody (AH1) [NBP1-05209]](https://resources.bio-techne.com/images/products/NF-H-Antibody-AH1-Western-Blot-NBP1-05209-img0005.jpg "Western Blot: NF-H Antibody (AH1) [NBP1-05209]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Equine
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # AH1
Concentration
1 mg/ml
Product Specifications
Immunogen
Native axonal phosphorylated Neurofilament Heavy protein purified from bovine spinal cord
Reactivity Notes
In some species there is some cross-reactivity with the phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken
Specificity
Clone AH1 recognizes phosphorylated NF-H KSP sequences. Does not recognize non-phosphorylated KSP sequences.
Marker
Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NF-H Antibody (AH1)
Western Blot: NF-H Antibody (AH1) [NBP1-05209]
Western Blot: NF-H Antibody (AH1) [NBP1-05209] - Analysis of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (NBP1-05209) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.![Immunocytochemistry/ Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209]](https://resources.bio-techne.com/images/products/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0003.jpg "Immunocytochemistry/ Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209]")
Immunocytochemistry/ Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209]
Immunocytochemistry/Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209] - Mixed neuron/glial cultures stained with NBP1-05209 (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L NB300-131 (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NBP1-05209 binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.![Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209]](https://resources.bio-techne.com/images/products/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0004.jpg "Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209]")
Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209]
Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209] - Analysis of rat cerebellum section stained with mouse mAb to pNF-H, NBP1-05209, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05209 antibody stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3/NeuN antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.Applications for NF-H Antibody (AH1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:1000
Immunohistochemistry Free-Floating
1:1000
Western Blot
1:10000
Application Notes
This 200kDa Neurofilament Heavy antibody is useful for Imunocytochemistry, Western Blot, and Immunohistochemistry (both IHC-P and IHC-Fr). Use in IHC-P reported in scientific literature (PMID:35835391).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NF-H
Long Name
Neurofilament Protein, Heavy Chain
Alternate Names
NEFH, NFH
Gene Symbol
NEFH
Additional NF-H Products
Product Documents for NF-H Antibody (AH1)
Product Specific Notices for NF-H Antibody (AH1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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