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NF-H Antibody (CL2671) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43795

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2671

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P12036, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NF-H Antibody (CL2671) - Azide and BSA Free

NF-H Antibody (CL2671) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-H Antibody [NBP3-43795]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP3-43795]

Staining of human cerebellum shows moderate immunoreactivity in Purkinje cells and neural fibers.
NF-H Antibody (CL2671) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-H Antibody [NBP3-43795]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP3-43795]

Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
NF-H Antibody (CL2671) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-H Antibody [NBP3-43795]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP3-43795]

Analysis in human cerebral cortex and tonsil tissues using NBP3-43795 antibody. Corresponding NF-H RNA-seq data are presented for the same tissues.

Applications for NF-H Antibody (CL2671) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-H

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for NF-H Antibody (CL2671) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NF-H Antibody (CL2671) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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