NF-H Antibody (NAP4)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-136

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Chicken, Equine

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NAP4

Concentration

1 mg/ml

View all NF-H Antibodies »

Product Specifications

Immunogen

Native NF-H purified from bovine spinal cord

Localization

Cytoplasmic

Specificity

Specifically recognizes the phosphorylated variant of NF-H subunit (~200-220 kDa), showing some weaker reactivity with phosphorylated forms of NF-M.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

200-220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-H Antibody (NAP4)

Western Blot: NF-H Antibody (NAP4) [NB300-136]

Western Blot: NF-H Antibody (NAP4) [NB300-136]

Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.
Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136]

Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136]

Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136] - Analysis of human cerebellar cortex section stained with mouse mAb to pNF-H, NB300-136, in brown. Paraffin-embedded, formalin-fixed tissue sections were stained with this antibody using the avidin biotin conjugate method. The sections was counterstained with Hematoxylin in blue. NB300-136 stains prominent basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image, the molecular layer at the top.

Applications for NF-H Antibody (NAP4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:10000
Application Notes
This 200-220 kDa Neurofilament Heavy antibody can be used for ELISA, Immunohistochemistry on paraffin-embedded sections and Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-H

Neuronal intermediate filaments (NIF) are found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 200kD neurofilament heavy (NEFH) is one of the major components of neuronal cytoskeleton NIF and NEFH gene was originally proposed as a DNA marker for presymptomatic diagnosis in neurofibromatosis type 2 (NF2). NEHF facilitate interactions between NIF and brain mitochondria with binding efficiency dependent upon mitochondrial membranes potential. NEFH and beta-catenin are constituents in postsynaptic density (PSD) in CNS and NEFH is essential for maintaining normal cell integrity. Diminished NEFH levels are seen in certain cancers including human autonomic nerve tumors or central neurocytomas, prostate cancer, clear-cell epithelioid tumor and small cell lung carcinoma. Fibroblasts overexpressing NEFH are distorted, multinucleated, and possess inclusions, typical changes seen in neuropathies, and defects in NEFH are linked to susceptibility to ALS.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

4744 (Human)

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for NF-H Antibody (NAP4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NF-H Antibody (NAP4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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