NF-H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37984

Novus Biologicals, part of Bio-Techne
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NBP2-37984
NBP2-37984-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NF-H Antibodies »

Product Summary for NF-H Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NF-H Antibody

Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984] - Staining of human liver shows no positivity as expected.
Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984]

Immunohistochemistry-Paraffin: NF-H Antibody [NBP2-37984] - Analysis in human cerebral cortex and liver tissues. Corresponding NF-H RNA-seq data are presented for the same tissues.
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Applications for NF-H Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-H

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Gene Symbol

NEFH

UniProt

P12036

Additional NF-H Products

Product Documents for NF-H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NF-H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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