NF-M Antibody (3H11)

Western Blot: NF-M Antibody (3H11) [NB300-134]

Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.

Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134]

Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134] - Adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M, dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-134 antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody stains the network of neuronal axons only.

Western Blot: NF-M Antibody (3H11) [NB300-134]

Western Blot: 160kDa Neurofilament Medium Antibody (3H11) [NB300-134] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NB300-134 (lane 3).