NF-M Antibody (5E0D2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15655
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5E0D2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600-700 of human NF-M (P07197). ELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NF-M Antibody (5E0D2)
![Western Blot: NF-M Antibody (5E0D2) [NBP3-15655]](https://resources.bio-techne.com/images/products/NF-M-Antibody-5E0D2-Western-Blot-NBP3-15655-img0002.jpg "Western Blot: NF-M Antibody (5E0D2) [NBP3-15655]")
Western Blot: NF-M Antibody (5E0D2) [NBP3-15655]
Western Blot: NF-M Antibody (5E0D2) [NBP3-15655] - Western blot analysis of extracts of various cell lines, using NF-M antibody (NBP3-15655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: NF-M Antibody (5E0D2) [NBP3-15655]](https://resources.bio-techne.com/images/products/NF-M-Antibody-5E0D2-Immunocytochemistry-Immunofluorescence-NBP3-15655-img0001.jpg "Immunocytochemistry/ Immunofluorescence: NF-M Antibody (5E0D2) [NBP3-15655]")
Immunocytochemistry/ Immunofluorescence: NF-M Antibody (5E0D2) [NBP3-15655]
Immunocytochemistry/Immunofluorescence: NF-M Antibody (5E0D2) [NBP3-15655] - Immunofluorescence analysis of SH-SY5Y cells using NF-M Rabbit mAb (NBP3-15655) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for NF-M Antibody (5E0D2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NF-M
Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Medium Chain
Alternate Names
NEF3, NEFM, NFM
Gene Symbol
NEFM
Additional NF-M Products
Product Documents for NF-M Antibody (5E0D2)
Product Specific Notices for NF-M Antibody (5E0D2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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