NF2/Merlin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03740

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NF2/Merlin Antibodies »

Product Summary for NF2/Merlin Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2/Merlin (NP_000259.1). TKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NF2/Merlin Antibody - BSA Free

Western Blot: NF2/Merlin AntibodyBSA Free [NBP3-03740]

Western Blot: NF2/Merlin AntibodyBSA Free [NBP3-03740]

Western Blot: NF2/Merlin Antibody [NBP3-03740] - Analysis of extracts of various cell lines, using NF2/Merlin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NBP3-03740]

Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NBP3-03740]

Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP3-03740] - Immunofluorescence analysis of U2OS cells using NF2/Merlin antibody (NBP3-03740) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NBP3-03740]

Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NBP3-03740]

Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP3-03740] - Immunofluorescence analysis of C6 cells using NF2/Merlin antibody (NBP3-03740) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Applications for NF2/Merlin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NF2/Merlin

Merlin, also known as Neurofibromin 2 (NF2), is a tumor suppressor gene. Merlin is closely related to the ERM (ezrin, radixin, moesin) family of proteins. The precise function of Merlin is not clear. It is thought to provide a link between the actin cytoskeleten and membrane associated proteins, playing a role in transduction of extracellular signals. Merlin has been implicated in cell proliferation and cellular motility.

Long Name

Neurofibromin 2

Alternate Names

ACN, BANF, Merlin, SCH, Schwannomerlin, Schwannomin

Gene Symbol

NF2

Additional NF2/Merlin Products

Product Documents for NF2/Merlin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NF2/Merlin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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