NFATC1/NFAT2 Antibody (7A6)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-620

Novus Biologicals, part of Bio-Techne
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NB300-620
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Primate, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7A6

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all NFATC1 Antibodies »

Product Specifications

Immunogen

Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 16129432). Hamster reactivity reported in scientific literature (PMID: 10866678). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects cytosolic nuclear factor of activated T-cells (NFAT 2) from transfected human, mouse, and monkey cells. This does not cross react with NFATp.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NFATC1/NFAT2 Antibody (7A6)

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human spleen tissue.
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Applications for NFATC1/NFAT2 Antibody (7A6)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:20 - 1:200

Immunohistochemistry-Paraffin

1:20 - 1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
WB: Detects an approx. 105 kDa band representing transfected NFAT2 from COS cell lysate.
See Simple Western Antibody Database for Simple Western validation: separated by size
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NB300-620 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites diluted with PBS

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFATC1

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Entrez Gene IDs

4772 (Human); 18018 (Mouse); 100361818 (Rat)

Gene Symbol

NFATC1

UniProt

O95644

Additional NFATC1 Products

Product Documents for NFATC1/NFAT2 Antibody (7A6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFATC1/NFAT2 Antibody (7A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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