NFATC2/NFAT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51970

Novus Biologicals, part of Bio-Techne
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NBP1-51970
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NFATC2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QQFRTGLSSPDARYQ corresponding to internal region according to NP_775114.1, NP_001129493.1.

Epitope

C-QQFRTGLSSPDARYQ

Specificity

This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NFATC2/NFAT1 Antibody

Western Blot: NFATC2/NFAT1 Antibody [NBP1-51970]

Western Blot: NFATC2/NFAT1 Antibody [NBP1-51970]

Western Blot: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-51970]

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-51970]

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NFATC2/NFAT1 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human MOLT4 lysates (calculated MW of 100 kDa band according to NP_775114.1). IHC-P: Human spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFATC2

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT1, NFATP

Entrez Gene IDs

4773 (Human); 140488 (Mouse); 312320 (Rat)

Gene Symbol

NFATC2

UniProt

NP_036472.2 (Human)

Additional NFATC2 Products

Product Documents for NFATC2/NFAT1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFATC2/NFAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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