NFIB Antibody (2D6)

Novus Biologicals, part of Bio-Techne | Catalog # H00004781-M08

Novus Biologicals, part of Bio-Techne
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H00004781-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFIB Antibodies »

Product Specifications

Immunogen

NFIB (AAH01283, 1 a.a. ~ 420 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHNDPAKNPPGYLEDSFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQSWYLG

Specificity

NFIB - nuclear factor I/B (2D6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NFIB Antibody (2D6)

Western Blot: NFIB Antibody (2D6) [H00004781-M08]

Western Blot: NFIB Antibody (2D6) [H00004781-M08]

Western Blot: NFIB Antibody (2D6) [H00004781-M08] - Analysis of NFIB expression in transfected 293T cell line by NFIB monoclonal antibody (M08), clone 2D6. Lane 1: NFIB transfected lysatE (47.4 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NFIB Antibody (2D6) [H00004781-M08]

Immunocytochemistry/ Immunofluorescence: NFIB Antibody (2D6) [H00004781-M08]

Immunocytochemistry/Immunofluorescence: NFIB Antibody (2D6) [H00004781-M08] - Analysis of monoclonal antibody to NFIB on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: NFIB Antibody (2D6) [H00004781-M08]

Immunohistochemistry-Paraffin: NFIB Antibody (2D6) [H00004781-M08]

Immunohistochemistry-Paraffin: NFIB Antibody (2D6) [H00004781-M08] - Analysis of monoclonal antibody to NFIB on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml

Applications for NFIB Antibody (2D6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFIB

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein

Entrez Gene IDs

4781 (Human)

Gene Symbol

NFIB

UniProt

AAH01283 (Human)

Additional NFIB Products

Product Documents for NFIB Antibody (2D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NFIB Antibody (2D6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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