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NFIB Antibody (7T1G5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16757

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16757-20ul
NBP3-16757-100ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7T1G5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 321-420 of human NFIB (NP_005587.2). KPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQSWYLG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFIB Antibody (7T1G5)

Western Blot: NFIB Antibody (7T1G5) [NBP3-16757]

Western Blot: NFIB Antibody (7T1G5) [NBP3-16757]

Western Blot: NFIB Antibody (7T1G5) [NBP3-16757] - Western blot analysis of extracts of various cell lines, using NFIB antibody (NBP3-16757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for NFIB Antibody (7T1G5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFIB

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein

Gene Symbol

NFIB

Additional NFIB Products

Product Documents for NFIB Antibody (7T1G5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFIB Antibody (7T1G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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